General and specific transcription factors. Transcription initiation complex & looping. Combinatorial regulation.
Log in Martina Sanjuan 7 years agoPosted 7 years ago. Direct link to Martina Sanjuan's post “Alright but I still don't...” Alright but I still don't understand what is the difference between a general transcription factor and a specific one. • (23 votes) Langerhans 4 years agoPosted 4 years ago. Direct link to Langerhans's post “General, or basal, transc...” General, or basal, transcription factors simply assist in the binding of RNA polymerase to the promoter. Other types of transcription factors include activators and repressors. These transcription factors affect transcription in different ways; activators assist in the binding of RNA polymerase and repressors stop transcription. (27 votes) MissMollyMay13 7 years agoPosted 7 years ago. Direct link to MissMollyMay13's post “can a single mRNA strand ...” can a single mRNA strand be translated multiple times? • (9 votes) Christian Krach 7 years agoPosted 7 years ago. Direct link to Christian Krach's post “Yes, it can even be read ...” Yes, it can even be read by several ribosomes at once. (24 votes) Jennifer Kim 6 years agoPosted 6 years ago. Direct link to Jennifer Kim's post “Are enhancers required fo...” Are enhancers required for transcription to occur? • (7 votes) Luca Lenzi 6 years agoPosted 6 years ago. Direct link to Luca Lenzi's post “Generally, enhancers can ...” Generally, enhancers can be bound by activators to increase the likelihood that a particular gene will be transcribed. Therefore, they are not strictly required. (8 votes) gulalai hussain 7 years agoPosted 7 years ago. Direct link to gulalai hussain's post “does prokaryotes have any...” does prokaryotes have any transcription factors? • (4 votes) Glori Das 7 years agoPosted 7 years ago. Direct link to Glori Das's post “Yes, prokaryotes have tra...” Yes, prokaryotes have transcription factors. Think about E. coli and the lac operon. The activator and repressor proteins involved in lac operon expression are the transcription factors. However, the mechanisms by which transcription factors work are simpler than those in eukaryotes. (10 votes) renirarugnath 6 years agoPosted 6 years ago. Direct link to renirarugnath's post “How do transcription fact...” How do transcription factors differ from sigma factors? • (8 votes) Ivana - Science trainee 5 years agoPosted 5 years ago. Direct link to Ivana - Science trainee's post “Well, apart from being pr...” Well, apart from being proteins to control transcription in Prokaryotes, they are hom*ologous to archaeal transcription factor B and to eukaryotic factor TFIIB. Sigma factors are also needed at the promoter to initiate transcription, while transcription factors regulate the gene expression. (1 vote) Nauman Khalid 7 years agoPosted 7 years ago. Direct link to Nauman Khalid's post “are all transcriptional f...” are all transcriptional factors proteins? if not what are different transcription factors? • (3 votes) Ryan Hoyle 7 years agoPosted 7 years ago. Direct link to Ryan Hoyle's post “Yes, all transcription fa...” Yes, all transcription factors are proteins. They are coded for by regulatory genes, which are genes that encode a protein involved in regulation of gene expression (such as a transcription factor). However, recently people are discovering that transcription factors can have bits of sugar and other non-protein stuff added to them to regulate their activity. But yes, all transcription factors are proteins. (4 votes) Junsang 5 years agoPosted 5 years ago. Direct link to Junsang's post “Does general transcriptio...” Does general transcription factors always bind to proximal control elements, and specific transcription factors to distal? Also, are the bindings to specific transcription factors essential for that individual gene to start transcription? I would very much appreciate the help. • (3 votes) tyersome 5 years agoPosted 5 years ago. Direct link to tyersome's post “Good question!While I b...” Good question! While I believe the pattern you describe (with the general transcription factors binding to proximal elements) is common, many promoters (possibly most) don't follow that pattern. For example, according to a 2014 review† only ~20% of RNA polymerase II promoters contain a TATA box (which means that ~80% aren't bound by TATA binding protein) and ~30% have no recognizable promoter elements! Another example is that many (but not all) genes transcribed by RNA polymerase III have promoters within the gene§. †Note: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214234/ §Note: See the first figure in this review for details: As for your second question, it appears that some "housekeeping"¶ genes (including many of the TATA-less pol II promoters) lack specific factor binding sites. ¶Note: "housekeeping" genes (e.g. translation factors and ribosomal proteins) are expressed everywhere and at a so their expression doesn't require a lot of fine tuning. (3 votes) Mary Beth Williams 5 years agoPosted 5 years ago. Direct link to Mary Beth Williams's post “Which ways would you test...” Which ways would you test if a mutant gene was affecting a transcription factor? • (3 votes) Ivana - Science trainee 5 years agoPosted 5 years ago. Direct link to Ivana - Science trainee's post “Knock-out a gene.Targeted...” Knock-out a gene.Targeted gene deletion in order to study the efefct of gene mutation. (2 votes) SULAGNA NANDI a year agoPosted a year ago. Direct link to SULAGNA NANDI's post “For the Tbx4 gene, how do...” For the Tbx4 gene, how does RNAP transcribe the whole gene for a lung cell? The lung cell enhancer is right in the middle of the gene. • (3 votes) Kevindu De Silva 6 years agoPosted 6 years ago. Direct link to Kevindu De Silva's post “Do molecules that bind to...” Do molecules that bind to repressors to change their shape count as transcription factors too? If not, what are they called? • (4 votes) Ivana - Science trainee 5 years agoPosted 5 years ago. Direct link to Ivana - Science trainee's post “Yeah, it could be transcr...” Yeah, it could be transcription activator. (0 votes)Want to join the conversation?
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http://genesdev.cshlp.org/content/16/20/2593.full
